Observations on the Efficacy of Acupuncture plus Medicine in Treating Senile Hepatic Cirrhosis / 上海针灸杂志

Objective To investigate the clinical efficacy of acupuncture plus medicine in treating senile hepatic cirrhosis.Methods Eighty patients with senile hepatic cirrhosis were randomized to treatment and control groups, 40 cases each. Both group received conventional antiviral and anti-inflammatory treatments. In addition, the control group took domperidone tablets and spironolactone tablets orally and the treatment group received acupuncture. Serum HBV-DNA content was measured, hepatic fibrosis indicators (typeⅢ procollagen, serum hyaluronic acid and laminin) were examined, and clinical symptoms (ascites, hydrothorax, lower limb edema and abdominal varices) and gastrointestinal symptoms (reflux vomiting disappearance time, borborygmus recovery time and defecation frequency) were observed in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups.Results The total efficacy rate was 95.5％ in the treatment group and 77.5％ in the control group; there was a statistically significant difference between the two groups (P<0.05). There were statistically significant pre-/post-treatment differences in serum HBV-DNA content and hepatic fibrosis indicators in the two groups (P<0.05). There were statistically significant post-treatment differences in serum HBV-DNA content and hepatic fibrosis indicators between the treatment and control groups (P<0.01). There was a statistically significant post-treatment difference in the number of cases of ascites, hydrothorax or lower limb edema between the treatment and control groups (P<0.05). There were statistically significant post-treatment differences in the gastrointestinal symptoms between the treatment and control groups (P<0.01).Conclusion Acupuncture plus medicine is an effective way to treat senile hepatic cirrhosis.

Evaluation of efficacy and safety of different doses Octreotide combined with Lansoprazole in the treatment of hepatocirrhosis with upper gastrointestinal bleeding / 药物评价研究

Objective To evaluate the efficacy and safety of different doses Octreotide combined with Lansoprazole in the treatment of hepatocirrhosis with upper gastrointestinal bleeding.Methods Eighty hepatocirrhosis patients with upper gastrointestinal bleeding during May.2013 to May.2016 were treated with Octreotide combined with Lansoprazole,and were divided into high dose group (40 cases) and low dose group (40 cases) according to different doses Octreotide.Before and after treatment,portalvenousmaximumvelocity (PVV) and portalveindiameter (PVD) were detected by color Doppler ultrasound respectively,and haemostatic time,blood transfusion cases and volume,rehaemorrhagia cases were recorded,and adverse reactions were observed.Results After treatment,PVV and PVD of the two groups were significantly ameliorative (P ＜ 0.05),and there were no significantly different between two groups.Haemostatic time,blood transfusiorate,blood transfusiorate volume and rehaemorrhagia rate of the high dose group were significantly less than the low dose group (P ＜ 0.05).The clinical effective rate of the high dose group was significantly higher than that of the low dose group (P ＜ 0.05).The rate of adverse reactions of the high dose group was not significantly different from that of the low dose group.Conclusions High doses Octreotide combined with Lansoprazole in the treatment of hepatocirrhosis with upper gastrointestinal bleeding have a gain on hemostatic effect,and cannot increase adverse reactions significantly.

Values of MRE in diagnosis of stages of hepaticfibrosis:A Meta-analysis / 吉林大学学报(医学版)

Objective:To investigate the efficacy and the clinical value of magnetic resonance elastography(MRE) in diagnosis of hepatic fibrosis with Meta-analysis, and to provide basis for clinical treatment of hepatic fibrosis.Methods:The studies published before February 2, 2017 about MRE and staging of hepatic fibrosis in Chinese or English were retrived in the databases including PubMed, EMBase, Web of Science, Cochrane Library,CNKI, CBMDisc,VIP, Wanfang data, and supplemented by manual retrieval for relevant literatures.The inclusion and exclusion criterions were used to select and extract the literatures.The literatures qualitie were valuated based on QUADAS-2 tool.The sensitivity(SEN), specificity (SPE), diagnostic odds ratio (DOR), positive likelihood ratio (+LR), negative likelihood ratio (-LR) on the groups of F0 vs F1-F4,F0-F1 vs F2-F4,F0-F2 vs F3-F4, F0-F3 vs F4 and heterogeneity were combined and tested with Stata software respectively.HSROC and AUROC were also implemented.Results:A total of 1 332 studies were searched, and 22 were included.21 of them were in English and 1 in Chinese.The results of Meta analysis showed that the SENp, SPEp, +LRp,-LRp, DOR and AUROC in F0 vs F1-F4 group were 88.8%(85.0-91.7),95.9%(91.5-98.0),21.435(10.215-44.979),0.117(0.086-0.159),183.187(72.533-462.650) and 0.96(0.94-0.98) ,respectively;the SENp, SPEp, +LRp,-LRp, DOR and AUROC in F0-F1 vs F2-F4 group were 93.3%(89.2%-35.9%), 94.1%(90.2%-96.5%),15.839(9.344-26.848),0.072(0.044-0.117),221.224(100.980-484.648) and 0.98(0.96-0.99),respectively;the SENp, SPEp, +LRp, -LRp, DOR and AUROC in F0-F2 vs F3-F4 group were 92.9%(88.9%-95.5%),94.6%(91.2%-96.8%),17.348(10.496-28.671),0.075(0.048-0.119),230.434(111.482-476.317)0.98(0.96-0.99), respectively;the SENp, SPEp, +LRp,-LRp, DOR and AUROC in F0-F3 vs F4 group were 97.7%(93.0%-99.3%),93.2%(90.3%-95.2%),14.337(9.910-20.742),0.025(0.008-0.075),580.405(144.871-2325.307) and 0.98(0.96-0.99),respectively.Conclusion:MRE,as a new and noninvasive imaging method, has high diagnostic value in all stages of hepatic fibrosis, which can provide a reliable reference for clinical precise treatment of hepatic fibrosis.

Influence of lactulose on interventional therapy for HCC patients with hepatocirrhosis and hypersplenism

OBJECTIVE@#To investigate the influence of lactulose on immunity of hepatocellular carcinoma (HCC) patients with hepatocirrhosis and hypersplenism after double-interventional therapies.@*METHODS@#A total of 40 HCC patients with hepatocirrhosis and hypersplenism, hospitalized during January 2013 to June 2014, were enrolled and randomized into control group and observation group. Both groups received partial splenic embolization combined with transcatheter arterial chemoembolization. Besides, observation group orally took lactulose 30 mL/d. Four days before interventional therapies and at days 1, 3, 7 and 14 after therapies, fasting venous blood was collected to detect white blood cell count, red blood cell count (RBC), and platelet count (PLT). Four days before therapies and at days 7 and 14 after therapies, the levels of alanine aminotransferase, aspartate transaminase, total bilirubin, malondialdehyde, super-oxide dismutase (SOD), IFN-γ, and IL-4 as well as the distribution of T cell subsets in peripheral blood were tested. Complications were observed after interventional therapies.@*RESULTS@#Before interventional therapies the levels of white blood cell count, PLT and RBC in both groups showed no difference, while after interventional therapies the levels of PLT and RBC in both groups showed an increasing tendency (P < 0.05). At day 14 after interventional therapies, the level of blood cell as well as that of SOD, IFN-γ and IL-4 in serum were significantly higher than that before therapies; meanwhile, the levels of alanine aminotransferase and total bilirubin of observation group after therapies were significantly lower than before and control group (P < 0.05), the levels of CD4(+)/CD8(+), SOD and IFN-γ were all higher than before and control group (P < 0.05).@*CONCLUSIONS@#Oral administration of lactulose could adjust the imbalance of oxidation system/antioxidant system in HCC patients with hepatocirrhosis and hypersplenism after interventional therapies, and improve the antitumor immunity and prognosis.

Research progress of helicobacter pylori in the development of hepatitis,cirrhosis and hepatic carcinoma / 中国综合临床

Objective Helicobacter pylori ( Hp ) infection is an important risk factor of the gastrointestinal disease, including chronic gastritis, peptic ulcer and gastric cancer. However, many recent experimental and clinical studies have shown that its addition to causing gastrointestinal diseases, but also associated with many diseases, and closely related with the occurrence of liver diseases. Summarized recent advances in the study of the relevant studies,including the relationship between hepatitis and cirrhosis,and the occurrence and development of liver cancer.

Study on pathomorphological changes of the Iiver in Beagle Dog with spontaneous hepatocirrhosis / 中国比较医学杂志

Objective To study the liver pathomorphological and serum biochemical changes in Beagle dog with spontaneous hepatocirrhosis and establish the backgroud information of experimental animals for GLP .Methods The ALT, AST,TP,ALB, ALP, TBIL, TC, TG and GGT were detected using an automatic biochemical analyzer , and compared the differences of above index between blank control and diseased animal .The histological changes of the liver were observed by optical microscopy.Results Compared with the blank control ,the ALT,AST,ALP,TBIL and GGT of diseased dogs were increased significantly , and the ALB decreased significantly .Compared with normal dogs , the liver cells had nodular regeneration , arranged irregularly and pseudolobule formation .The pseudolobules were packaged with collagen fibers . Conclusion It is suggested that spontaneous lesions in Beagle dogs should be monitored so as to provide appropriate experimental animal histopathologicalbackgroud information for drug safety evaluation .

Determination of Serum Concentration of Vitamin A in Healthy Volunteers and Hepatocirrhosis Patients by HPLC / 中国药房

OBJECTIVE:To establish a method for the concentration determination of vitamin A(VitA)in human serum,and apply it in healthy volunteers and hepatocirrhosis patients. METHODS:After liquid-liquid extraction,serum sample was deter-mined by HPLC. Using VitA acetic ester as internal standard,the separation was performed on Kromasil C18 column with mobile phase consisted of methanol-water (98∶2,V/V) at the flow rate of 1 ml/min. The wavelength was set at 325 nm,and the column temperature was room temperature. The sample size was 20 μl. RESULTS:The linear range of VitA were 0.012 4-3.210 μg/ml(r=0.997 2,n=5),and the limit of quantification was 0.012 4 μg/ml. Inter-day and intra-day RSD ranged 1.66%-2.97%;sample re-coveries were 98.18%-99.56%;extraction recoveries were 89.59%-91.38%. Average blood concentration of VitA were(0.71±0.08)μg/ml in 24 healthy volunteers and (0.28 ± 0.06)μg/ml in 24 hepatocirrhosis patients. There was statistical significance in average blood concentration of VitA between healthy volunteers and hepatocirrhosis patients in different age groups (P<0.05). CONCLU-SIONS:The method is simple,rapid,sensitive and accurate,and can be used for the concentration determination of VitA in serum of healthy volunteers and hepatocirrhosis patients.

Effects of Bifid Triple Viable Capsules Combined with Dahuang Chishao Decoction on Endotoxemia and Intestinal Mucosal Barrier Function of Patients with Hepatocirrhosis / 中国药房

OBJECTIVE:To discuss effects of Bifid triple viable capsules combined with Dahuang chishao decoction on endo-toxemia and intestinal mucosal barrier function of patients with hepatocirrhosis. METHODS:90 patients with hepatocirrhosis were randomly divided into traditional Chinese and western medicine group and western medicine group with 45 cases in each group. Both groups were given symptomatic treatment,such as liver protection and diuresis,lowering portal pressure and nutrition sup-port. Based on this,western medicine group was given Bifid triple viable capsules 0.42 g,tid,po;traditional Chinese and western medicine group was additionally given Dahuang chishao decoction orally,one dose a day,on the basis of western medicine group. Both 2 groups were treated for 6 weeks. The changes of liver function index,serum endotoxin(ET),procalcitonin(PCT)and di-amine oxidase (DAO) levels of patients were observed and recorded in 2 groups before and after treatment. RESULTS:After 6 weeks treatment,serum ALT,AST and TBIL,ET,PCT and DAO levels of patients in 2 groups declined significantly,compared with before treatment,with statistical significance(P<0.05 or P<0.01);the decrease of traditional Chinese and western medicine group was more significant than western medicine group,with statistical significance(P<0.05). CONCLUSIONS:Bifid triple via-ble capsules combined with Dahuang chishao decoction can reduce ALT,AST and TBIL levels in patients with hepatocirrhosis,pro-tect liver function;reduce serum ET,PCT and DAO levels,release endotoxemia,and protect and remedy intestinal mucosal barri-er function.

Effects of dexmedetomidine on the perioperative stress response and immunologic function in hepatectomy patients with hepatitis cirrhosis / 临床麻醉学杂志

Objective To observe the effects of dexmedetomidine on the perioperative stress response and immunologic function in hepatectomy patients with hepatitis cirrhosis.Methods Forty patients(Child-Pugh A or B),ASA Ⅱ or Ⅲ with hepatitis cirrhosis undergoing hepatectomy were randomly divided into dexmedetomidine group(group D)and control group(group C)with 20 cases in each.Patients in group D were administered 0.5 μg/kg bolus dose of dexmedetomidine over 10 min, followed by 0.4 μg·kg-1 ·h-1 infusion until closing abdomen.And patients in group C were given normal saline by the same way as in group D.Flow cytometry was used to detect peripheral blood T lymphocytes (CD3 + ,CD4 + ,CD8 + ,CD4 +/CD8 + ),NK cells at 30 min before anesthesia(T0 ),imme-diately(T1 ),24 h(T2 )and 48 h(T3 )after surgery.Enzyme-linked immunosorbent assay was used to detect the levels of inflammatory cytokines concentration(IL-2,IL-10)at T0-T3 and stress hormones (Cor,ACTH,ALD)at T0 ,T2 and T3 .The levels of SBP,DBP and CVP at T0 ,immediately with intu-bation(Ta ),T1 and extubation(Tb ).Results Compared to those at T0 ,the levels of CD3 + ,CD4 + , CD8 + ,CD4 +/CD8 + at T1 and T2 were significantly lower in both groups(P <0.05),and the levels in group D were obviously higher than those in group C(P <0.05).The levels in group D were back to preoperative levels at T3 ,but the levels in group C were still lower than those at T0 (P <0.05).The levels of NK cells were higher at T1 in both groups,and the levels in group D was significantly higher than those in group C(P <0.05).Compared to those at group D,the concentration of IL-2 was lower at T1 and T2 ,the concentration of IL-10 was higher at T2 and T3 in group C (P <0.05).The levels of Cor,ALD,ACTH at T2 and T3 were significantly higher than those at T0 in both groups,but those in group D were markedly lower than those in group C (P < 0.05 ).The SBP,DBP and CVP of two groups was no statistically significant difference at T0 ,and SBP,DBP and CVP of the group D at Ta , T1 ,Tb was lower than that of T0 and group C (P <0.05).Conclusion Patients with hepatitis cirrho-sis underwent general anesthesia has certain immune suppression after liver cancer surgery,and con-tinuous intravenous infusion of dexmedetomidine during anesthesia may suppress the perioperative stress reaction effectively and alleviate the inhibition of immunologic function.

Influence of lactulose on interventional therapy for HCC patients with hepatocirrhosis and hypersplenism

Objective: To investigate the influence of lactulose on immunity of hepatocellular carcinoma (HCC) patients with hepatocirrhosis and hypersplenism after double-interventional therapies. Methods: A total of 40 HCC patients with hepatocirrhosis and hypersplenism, hospitalized during January 2013 to June 2014, were enrolled and randomized into control group and observation group. Both groups received partial splenic embolization combined with transcatheter arterial chemoembolization. Besides, observation group orally took lactulose 30 mL/d. Four days before interventional therapies and at days 1, 3, 7 and 14 after therapies, fasting venous blood was collected to detect white blood cell count, red blood cell count (RBC), and platelet count (PLT). Four days before therapies and at days 7 and 14 after therapies, the levels of alanine aminotransferase, aspartate transaminase, total bilirubin, malondialdehyde, super-oxide dismutase (SOD), IFN-γ, and IL-4 as well as the distribution of T cell subsets in peripheral blood were tested. Complications were observed after interventional therapies. Results: Before interventional therapies the levels of white blood cell count, PLT and RBC in both groups showed no difference, while after interventional therapies the levels of PLT and RBC in both groups showed an increasing tendency (P < 0.05). At day 14 after interventional therapies, the level of blood cell as well as that of SOD, IFN-γ and IL-4 in serum were significantly higher than that before therapies; meanwhile, the levels of alanine aminotransferase and total bilirubin of observation group after therapies were significantly lower than before and control group (P < 0.05), the levels of CD4

Effect of telbivudine on serum IL-18,IL-12, IL-37 and TNF-αlevels in patients with HBV-related hepatocirrhosis / 中国生化药物杂志

Objective To explore the effect of telbivudine on serum interleukin-18(IL-18),IL-12, IL-37 and tumor necrosis factor α(TNF-α) levels in patients with HBV-related hepatocirrhosis.Methods 66 cases of patients with HBV-related hepatocirrhosis were collected and divided into treatment group and control group according to the different treatment method , 34 cases in control group were treated with routine treatment , 32 cases in treatment group were treated by telbivudine on the basis of routine treatment.The serum IL-18, IL-12, IL-37, TNF-αlevels and adverse reactions were compared post-treatment.Results Compared with control group, the levels of IL-18, IL-12, IL-37 and TNF-αdecreased significantly post-treatment (P<0.05).The above levels were significantly lower in treatment group post-treatment than that in control group(P<0.05).Conclusion Telbivudine adjuvant therapy could suppress viral replication, and improve liver function and immune response in patients with HBV-related hepatocirrhosis.

Therapeutic Observation of Auricular Point Sticking with Magnetic Bead for Insomnia in Hepatocirrhosis / 上海针灸杂志

Objective To observe the clinical efficacy of auricular point sticking with magnetic bead in treating insomnia in hepatocirrhosis patients. Method Ninety patients with hepatocirrhosis complicated with insomnia were randomized into a treatment group and a control group, 45 in each group. The treatment group was intervened by auricular point sticking with magnetic bead, while the control group was by auricular point sticking with medical adhesive tape. After successive 2-week treatments, the Insomnia Severity Index (ISI) and the Evaluation Criteria of Therapeutic Efficacy for Mental Disorders were observed for evaluating the treatment result. Result The ISI was significantly improved in the treatment group after intervention (P<0.05), and the improvement was significantly different from that in the control group (P<0.05). The total effective rate was 86.7% in the treatment group versus 60.0% in the control group, and the difference was statistically significant (P<0.01). Conclusion Auricular point sticking with magnetic bead is easy-to-operate and can produce a satisfactory efficacy in treating insomnia of hepatocirrhosis patients.

The changes and significance of T lymphocytes subsets in diseases related to HBV infection / 国际检验医学杂志

Objective To investigate clinical significance of the changes in the peripheral blood T lymphocytes subsets in patients with diseases related to HBV infection .Methods 257 cases of inpatients and outpatients were selected from Jan .to Dec .2013 ,and were divided into hepatitis B carriers (ASC)group ,chronic hepatitis B(CHB)group ,hepatocirrhosis(LC)group and primary liver cancer(PHC)group according to types of diseases related to HBV infection .Other 50 healthy individuals conducted physical exami‐nation were enro1led in the control group .The absolute CD3+ ,CD4+ and CD8+ T‐cell count ,and CD3+ ,CD4+ and CD8+ percent‐age and CD4+ /CD8+ value were detected in all subjects by using flow cytometer .These data were compared and analyzed .Results Compared with the control group ,there were no significant differences of the absolute CD3+ ,CD4+ and CD8+ T‐cell count ,and CD3+ ,CD4+ and CD8+ percentage and CD4+ /CD8+ value in ASC group ,CHB group and LC group(P>0 .05) .Compared with the control group ,the absolute CD4+ T‐cell count ,CD4+ percentage and the CD4+ /CD8+ value were decreased in the PHC group , while the CD8+ percentage were increased in the PHC group ,there were statistical significant differences between the two groups (P<0 .05) .Conclusion The peripheral blood T lymphocyte subsets could be monitor indexes of cell immune function in diseases related to HBV infection .

Clinical significance of changes of lipid in elderly patients with hepatic schis-tosomiasis / 中国血吸虫病防治杂志

Objective To explore the clinical significance of lipid levels including total cholesterol TC triglyceride TG high?density lipoprotein HDL?C low density lipoprotein LDL?C and apolipoprotein APOAⅠand APOB of elder?ly patients with hepatic schistosomiasis. Methods A total of 280 hospitalized elderly patients with hepatic schistosomiasis 198 cases of chronic liver fibrosis and 82 cases of hepatocirrhosis were chosen as study objects and their clinical data were collected and analyzed retrospectively. Meanwhile the lipid levels between the patients with liver fibrosis and hepatocirrhosis and those among the patients with ABC degrees of Child?pugh grading of liver function were compared. Results Among the 280 patients the abnormality rates of the lipid levels were 34.8% 69/198 and 100% 82/82 in the liver fibrosis group and he?patocirrhosis group respectively and the difference between them were statistically significant χ2=5.74 P 0.05 . The levels of TC HDL?C LDL?C APOAⅠof the patients in the latter group were significantly lower than those in the former group all P 0.05 . The levels of TC TG HDL?C APOAⅠ APOB of the patients with C degree liver function were significantly lower than those of the patients with A degree liver function and the levels of TC TG HDL?C of the former were also lower than those of the patients with B degree liver function all P 0.05 . Conclusions The lipid levels of the elderly patients with he?patic schistosomiasis reduce obviously in the course of hepatocirrhosis and it is correlated with the damage level of the liver. Lipid and apolipoprotein detections have certain values on the illness judgment and prognosis assessment.

HBV-specific T lymphocyte responses during different stages of hepatitis B virus infection / 中华微生物学和免疫学杂志

Objective To analyze hepatitis B virus ( HBV)-specific T lymphocyte responses dur-ing different stages of HBV infection.Methods Eighty-four patients with HBV infection were recruited in this study.They were divided into four groups including acute HBV infection group (8 cases), chronic HBV infection group (39 cases), hepatocirrhosis group (17 cases) and hepatocellular carcinoma group (20 ca-ses) .HBV-specific T cell responses were detected by using ELISPOT assay in combination with magnetic beads sorting assay.Results (1)The magnitudes of HBV-specific T cell responses in patients with acute HBV infection ,chronic HBV infection , hepatocirrhosis and hepatocellular carcinoma were respectively (2067.00±1029.00) SFU/106 PBMCs, (288.50±57.69) SFU/106 PBMCs, (96.25±31.06) SFU/106 PBMCs and (71.47±14.26) SFU/106 PBMCs.The differences with the magnitudes of HBV-specific T cell responses among patients from the four groups were significant (P<0.01).(2)HBV Core (HBV C) protein induced the strongest T cell responses[ (323.90±130.30) SFU/106 PBMCs] in patients with acute HBV infection in comparison with HBV-surface ( HBV S ) protein, HBV P protein and HBV X protein ( P=0.0037).The strongest T cell responses in patients with chronic HBV infection were induced by using HBV P protein [(127.20±54.42) SFU/106 PBMCs], followed by using HBV S protein, HBV C protein and HBV X protein (P=0.0159).(3)The magnitudes of IFN-γreleasing induced by HBV X protein, HBV P protein, HBV S protein and HBV C protein showed no significant differences in patients with either hepato-cirrhosis or hepatocellular carcinoma, but were lower than those induced in patients with chronic HBV infec-tion.Conclusion HBV-specific T cell responses were gradually reduced along the progression of HBV in-fection from acute HBV infection to chronic HBV infection, liver cirrhosis and hepatocellular carcinoma.The HBV-specific T cell responses induced by viral proteins might play different roles in different stages of HBV infection.

Research progress of liver regeneration after partial hepatectomy / 中华肝胆外科杂志

Partial hepatectomy (PH) is widely used and the preferred method for the surgical treatment of hepatocellular carcinoma,and liver regeneration is directly related to the prognosis of the patients after the operation.Therefore,the specific mechanism and cytokines related to liver regeneration have become a hot topic in recent years.Currently,there is a wide variation of reported gene expressions and signal transduction pathways in the literature,but the mechanism and interactions are still unclear,especially for postoperative liver regeneration with hepatitis or cirrhosis.This review summarizes current research on the liver regeneration process,the mechanism of liver regeneration after partial hepatectomy,and the different mechanisms of hepatocirrhosis.

The study of the angitensin-converting enzyme gene polymorphism in hepatorenal syndrome / 中国基层医药

Objective To investigate the insertion/deletion(I/D) polymorphism of ACE gene in uncompen-sated hepatocirrhosis with hepatorenal syndrome(HRS). Methods ACE I/D polymorphism was detected by poly-merase chain reaction amplification of DNA fragment in 96 cases of uncompensated bepatocirrhnsis with HRS, every group as controls were involved in this study. At the same time,GPT,GOT,sarum creatinine(SCr), blood urea nitro-gen(BUN) and glomerular filtration rate(GFR) of every case were measured and the difference of these targets among different genotypes were compared. Results There was no significant difference for every genotype and allele fre-quency between the HRS group and every group as controls(P >0.05). The I allele frequencies were higher than the one of D allele for every groups except other liver disease group(P < 0.01). But in the every group as controls, there was no significant difference for the genotype frequency among three groups and in the HRS group, the Ⅱ genotype frequency was higher than the one of ID and DD(P < 0.05). SCr etc. of the Ⅱ genotype were higher than the one of ID and DD(P<0.05) and GFR of the Ⅱ genotype was lower than the one of ID and DD in the HRS group(P<0.05). Conclusion There was relationship between ACE gene polymorphism and the incidence of uncompensated bepatocirrhosis with HRS.

Complex Bacteria Infections in Severe Hepatitis and Hepatocirrhosis / 中华医院感染学杂志

OBJECTIVE To investigate the susceptible factors and pathogenic bacteria in severe hepatitis and hepatocirrhosis cases infected by complex bacteria.METHODS We reviewed the severe hepatitis and hepatocirrhosis cases admitted to our hospital during 2004-2007,who infected by complex bacteria,to investigate the risk factors and pathogenic bacteria.RESULTS In 216 cases of severe hepatitis and hepatocirrhosis,27 cases were infected by complex bacteria(12.5%).After testing in 66 strains of bacteria,there were 22 strains of Gram-negative bacteria(33.3%),10 strains of Gram-positive bacteria(15.2%),and 34 strains of fungi(51.5%),most of the cases were at terminal stage,with long-term hospitalization,with different underlying diseases,repeated use of broad-spectrum antibiotic and hormone,and invasive operations.All of the factors might induce the complex bacteria infections.CONCLUSIONS The complex bacteria infections in the severe hepatitis and hepatocirrhosis cases are critical and difficult to cure.The underlying diseases should be treated actively;the detection of pathogenic bacteria and drug sensitive test should be carried out;the strict measurement of disinfection and isolation are strongly suggestedto decrease the rate of infection of bacteria and prevent the hazards.

Quantitative analysis of liver function with contrast enhanced ultrasonography of liver cirrhosis / 中国医学影像技术

Objective To observe the corelation between quantitative index and the grade of the liver function with contrast-enhanced ultrasonography (CEUS) in hepatic cirrhosis. Methods Seventy-five patients of cirrhosis after HBV were divided into 3 groups (each n=25) on the basis of the liver function by Child-pugh method, while 20 healthy subjects were enrolled in the control group. The inner diameter and Doppler spectrum of right hepatic vein (RHVD, RHVF) were measured. After intravenous bolus injection 1.2 ml SonoVue, RHA and RHV were observed continually on real time, hepatic artery transit time (HATT) and hepatic vein transit time (HVTT) were recorded, then HTTs were calculated. Results In hepatic cirrhosis groups, RHVD decreased remarkably, RHVF was abnormal, HVTT and HTT were obviously shorter, and HTTs were shortened significantly.Conclusion CEUS may play an important role in qualitative evaluation of liver function, and HTT is more valuable than HVTT.

Detections and relevance of Ghrelin and inflammatory cytokines in peripheral blood of patients with chronic hepatic diseases / 中国免疫学杂志

Objective:The purpose of this study is to determine levels of Ghrelin,TNF-α,IL-1 and IL-6 in peripheral blood of patients with chronic hepatitis B,liver cirrhosis,and non-alcoholic fatty liver disease which diagnosed at different extent of damage,and based on it to have a further study on the relevance between Ghrelin and inflammatory cytokines in peripheral blood in above diseases.Methods:Ghrelin,TNF-α,IL-1 and IL-6 in peripheral blood were determined by ELISA method.Results:In patients with chronic hepatitis B andhepatic cirrhosis,Ghrelin level increased significantly as compared with that in normal control group (P＜0.05,P＜0.01).Ghrelin levels in decompensated liver cirrhosis (degree B and C)groups were much higher than in chronic hepatitis B group (P＜0.05). Ghrelin level in group of non-alcoholic fatty liver disease decreased significantly as compared with that in normal control group (P<0.01). Ghrelin and inflammatory cytokines,including TNF-α,IL-1 and IL-6 showed a positive relevances in groups of chronic hepatitis B and liver cirrhosis,but not in NAFLD group which showed a negative correlations.Conclusion:Ghrelin has a higher level in peripheral blood of patients with chronic hepatitis B and liver cirrhosis,but it has a lower level in patients with non-alcoholic fatty liver disease,suggesting correlation of Ghrelin level with the occurrence and the procession of chronic liver diseass.